NpFlamma® MMP-13 749 is a NIR dye-incorporated MMP-activatable chitosan nanoparticle (CNP) that enables to selectively detect tumor cells. The probe comprises Flamma® Fluor 749 dye that connected to a quencher through a MMP-13 cleavable peptide, and the peptide is chemically conjugated to CNP. Flamma® Fluor dyes display strong absorption, high fluorescence quantum yield and high photostability, and they maintain good fluorescence activity and stability after conjugated to biomolecules. The maxima of Ex/Em values are at 760/778 nm. BioActs offers NpFlamma® MMP-13 749 as a smart fluorescent probe for monitoring MMP-related diseases such as cancer progression, invasion and metastasis, rheumatoid arthritis, pulmonary diseases and areas of cardiovascular disease, and also for evaluating the potential therapeutic efficacy of drugs targeting for these diseases.
1.Optimization of matrix metalloproteinase fluorogenic probes for osteoarthritis imaging (Ju Hee Ryu, Aeju Lee, Jin Hee Na, Seulki Lee, Hyung Jun Ahn, Jong Woong Park, Cheol-Hee Ahn, Byung-Soo Kim, Ick Chan Kwon, Kuiwon Choi, Inchan Youn, Kwangmeyung Kim, Amino Acids, 2011, Volume 41, Issue 5, pp 1113–1122)
2.Dark Quenched Matrix Metalloproteinase Fluorogenic Probe for Imaging Osteoarthritis Development in Vivo (Seulki Lee, Kyeongsoon Park, Seung-Young Lee, Ju Hee Ryu, Jong Woong Park, Hyung Jun Ahn, Ick Chan Kwon, In-Chan Youn, Kwangmeyung Kim, Kuiwon Choi, Bioconjugate Chem., 2008, 19 (9), pp 1743–1747)
3.Early Diagnosis of Arthritis in Mice With Collagen-Induced Arthritis, Using a Fluorogenic Matrix Metalloproteinase 3–Specific Polymeric Probe (Ju Hee Ryu, Aeju Lee, Jun-Uk Chu, Heebeom Koo, ChangYong Ko, Han Sung Kim, Soo-Young Yoon, Byung-Soo Kim, Kuiwon Choi, Ick Chan Kwon, Kwangmeyung Kim, Inchan Youn, ARTHRITIS & RHEUMATISM, 2011, Vol. 63, No. 12, pp 3824–3832)
4.Polymeric Nanoparticle-Based Activatable Near-Infrared Nanosensor for Protease Determination In Vivo (Seulki Lee, Ju Hee Ryu, Kyeongsoon Park, Aeju Lee, Seung-Young Lee, In-Chan Youn, Cheol-Hee Ahn, Soon Man Yoon, Seung-Jae Myung, Dae Hyuk Moon, Xiaoyuan Chen, Kuiwon Choi, Ick Chan Kwon, Kwangmeyung Kim, Nano Lett., 2009, Vol. 9, No. 12, pp 4412-4416)