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Fluorescent NIR Dyes

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BioActs developed AngioFlamma® series as effective fluorescent probes for the detection of angiogenesis and tumor cells.

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BioActs developed CytoFlamma® cell membrane series as versatile membrane selective fluorescent probes.

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MitoFlamma® Green is a mitochondria-selective green fluorescent dye that allows to detect mitochondrial morphology in living cells.

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BioActs developed two series of apoptosis specific small peptide based fluorescent probes, ApoFlamma® H and ApoFlamma® PS, for effective monitoring of apoptosis.

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BioActs developed NpFlamma® MMP series, MMP-activatable polymeric in vivo fluorescent probes, for visualization of overexpressed MMPs related diseases.

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FLAMMA® FLUORS NHS ESTER

BioActs offers a broad range of Flamma® Fluors dyes equipped with variety of reactive and functional groups, which can cover the full spectral range from UV to NIR with their excellent fluorescence performance. Succinimidyl (NHS) esters are reliable activating moiety for amine labeling due to the formation of the strong amide bond.

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Flamma® 496 NHS ester

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Flamma® 552 NHS ester

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Flamma® 581 NHS ester

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Flamma® 648 NHS ester

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Flamma® 675 NHS ester

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Flamma® 749 NHS ester

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FLAMMA® NA NHS ESTER

BioActs offers Flamma NA series dyes for post synthetic fluorescence labeling method. Fluorescent oligonucleotide probes are developed for the detection and the quantification of target DNA or RNA sequence with the high sensitivity and specificity along with low toxicity.

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Flamma® 488NA NHS ester

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Flamma® 552NA NHS ester

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Flamma® 594NA NHS ester

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Flamma® 648NA NHS ester

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Flamma® 675NA NHS ester

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Flamma® 749NA NHS ester

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FLAMMA® FLUORS VINYLSULFONE

BioActs offers a broad range of Flamma® Fluors dyes equipped with variety of reactive and functional groups, which can cover the full spectral range from UV to NIR with their excellent fluorescence performance. BioActs’ leading technology developed highly reactive yet stable and pH insensitive Flamma® Fluors Vinylsulfone dyes. 

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Flamma® 488 Vinylsulfone

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Flamma® 552 Vinylsulfone

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Flamma® 594 Vinylsulfone

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Flamma® 648 Vinylsulfone

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Flamma® 675 Vinylsulfone

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Flamma® 749 Vinylsulfone

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<How Are Fluorescent Dyes Used in Biological Study?>

* Fluorescent dyes are organic molecules that absorb light and re-emit it at a longer wavelength. They are smaller and more photostable than fluorescent proteins, which makes them excellent candidates for labeling biomolecules. They are powerful tool for measuring cellular activity and properties in live cells, and by labeling with dyes, you can convert non-fluorescent compounds to fluorescent species.

[Fluorescent Dyes]

Fluorescence labeling of biomolecules with fluorescent dyes enable to visualize a tiny amount of labeled molecule from a complex mixture of biomolecule. Metal binding fluorescent dyes allow to directly measure non-fluorescent metals such as calcium, magnesium, zinc, copper, etc. Additionally, fluorescent dyes and quenchers are equipped with wide variety of reactive and functional groups.
Fluorescent dyes are widely used in observation and staining purposes, but conditions are required for their effective performance.
- They should be worked in the spectral range from UV to near infrared wavelength.
- Using them should not disturb the function of the molecule.
- The dye should be labeled in non-active site.

[FSD™ Fluor]

FSD Fluor™ is a new generation of dye series with superb fluorescence intensity and high quantum yield comparing to traditional dyes. The fluorescence intensity after binding to biomolecules such as antibody, nucleotide, and protein maintains still excellent. FSD Fluor™ series is ideal for variety of biochemical and biological analytical applications with a less amount of dye conjugate. FSD Fluor™ series equipped with a variety of reactive groups and covers the full fluorescence spectral range from UV to NIR. FSD Fluor™ dyes are suitable for every filter and are designed to meet the requirements for complex detection in the field of life science research.

[Flamma Fluors]

Flamma® Fluors dyes can cover the full spectral range from UV to NIR. Features of them are strong absorption, high fluorescence quantum yield and high photostability. Flamma® dyes maintain good fluorescence activity and stability after conjugation to biomolecules and allow the detection of low-abundance biological structures with great sensitivity. The dyes are compatible with optical conditions of most of fluorescent equipment and are ideal for any applications in biological studies. They are equipped with a variety of reactive groups: NHS and Sulfo-NHS ester, vinylsulfone, maleimide, click chemistry, isothiocyanate, and hydrazide.

[TAMRA and FAM]

5(6)-Carboxyfluorescein (FAM) and 5(6)-Carboxytetramethylrhodamine (TAMRA) dyes are used for labeling of biomolecules. FAM is one of popular green fluorescent reagents used for labeling peptides, proteins and nucleotides. In addition to relatively high absorptivity, good fluorescence quantum yield and good water solubility, FAM has an excitation maximum that closely matches the 488 nm spectral line of the argon-ion laser. TAMRA fluorophore has been a widely used for preparing bioconjugates, especially fluorescent antibody and avidin derivatives. TAMRA dye is also widely utilized for oligonucleotide labeling and automated DNA sequencing applications. TAMRA is often used as FRET acceptor for FAM fluorophore.

[qFlamma Quenchers]

qFlamma® quencher absorbs excitation light energy from a fluorophore and dissipates the energy as heat without any native fluorescence. The main advantage of the quencher is the non-emissive excitation and elimination of background fluorescence. In conjunction with fluorophores, qFlamma® quencher is a key component in designing FRET probes, in protease activity assays, nucleic acid hybridization, PCR, etc. The absorbance peaks of qFlamma® quenchers completely cover spectral range from visible to near-infrared that all of commercially available fluorescent dyes can be effectively quenched.

[High-Quality Fluorescent Dyes]

Since established in 1976, BioActs has developed and produced high quality of functional dyes and fluorescent biomaterials for a variety of applications such as fluorescent optical imaging probes for in vitro and in vivo study, near-infrared dyes for biomedical diagnostics, and contrast agents for small animal imaging. Our innovative research and service capabilities, combined with deep market knowledge and expertise, help customers gaining accurate insights to advance research. BioActs also offers a broad range of fluorescent and functional PS beads, magnetic beads, and nucleic acid labeling dyes. Additionally, BioActs provides a wide range of custom services, including fluorescence labeling, organic synthesis, oligonucleotide synthesis and fluorescent labeling.

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