Cell Structure Analysis Probes

MitoFlamma^® series

MitoFlamma^® series is a mitochondria-selective fluorescent dye that allows to detect mitochondrial morphology in living cells. This unique dye appears to preferentially accumulate in mitochondria regardless of mitochondrial membrane potential in certain cell types, making it a possible tool for determining mitochondrial mass. MitoFlamma^® series enables researchers to observe mitochondrial activity, localization and abundance as well as monitoring the effect of drugs or other external stimuli on the mitochondrial function. Once mitochondria are labeled with this special dye, the cells can be treated with an aldehyde fixative for samples that require fixation for further processing. MitoFlamma^® series probes are retained in the mitochondria during the fixation step and after permeabilization with some detergents during subsequent processing steps. After fixation, labeled samples can be applied in a variety of experiments such as immunocytochemistry, in situ hybridization, microplate-based analysis, etc.

Figure 1.   Imaging of MitoFlamma^® Green and MitoFlamma^® Deep Red mitochondria staining