ICG Thiol is an inactive form of near infrared (NIR) fluorescent dye and used to generate a stable fluorescence signal in bioimaging. The thiol is attached to ICG fluorophore trough a spacer. NIR fluorescence allows to observe the deep image from the surface of skin and being utilized in a wide range of research fields. The maxima of Ex/Em values are at 785/812 nm. ICG might be excited using 750-800 nm laser line or LED and displays excellent optical property. ICG thiol can be labeled to biomolecules through a disulfide bond formation with thiol of cysteine residue or can be utilized as a reference standard for dye-conjugates.
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