ICG Amine is an inactive form of near infrared (NIR) fluorescent dye and used to generate a stable fluorescence signal in bioimaging. The primary amine is attached to ICG fluorophore through a spacer. NIR fluorescence allows to observe the deep image from the surface of skin and being utilized in a wide range of research fields. The maxima of Ex/Em values are at 785/812 nm. ICG might be excited using 750-800 nm laser line or LED and displays excellent optical property. ICG amine can be coupled with carboxylic acids at small molecules or on biomolecules by standard amide bond coupling conditions or can be utilized as a reference standard for dye-conjugates.
1. Masashi Gotoh. Development of a canine model of pulmonary emphysema and imaging of the emphysematous lung with infrared thoracoscopy. J Thorac Cardiovasc Surg 126.6 (2003): 1916-21.
2. Aaron M. Mohs. An integrated widefield imaging and spectroscopy system for contrast-enhanced, image-guided resection of tumors. IEEE Trans Biomed Eng 62.5 (2015): 1416-24.
3. Mohammed Hassan. Near Infrared Fluorescence Imaging with ICG in TECAB Surgery Using the da Vinci Si Surgical System in a Canine Model. J Card Surg 27.2 (2012): 158-162.
4. R. C. Benson. Fluorescence properties of indocyanine green as related to angiography. Phys Med Biol 23.1 (1978): 159-63.
5. Mitsuharu Miwa. The Principle of ICG Fluorescence Method. The Open Surgical Oncology Journal 2 (2010): 26-28.