TAMRA Hydrazide

Catalog No. KWH1025


Lead time
1 mg $121.00
5 mg $279.00
25 mg $552.00
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TAMRA Hydrazide is a reactive form of bright yellow dye that used to generate a stable fluorescence signal in bioimaging. The maxima of Ex/Em values are at 544/576 nm, similar to that of DyLight 549, ATTO 550 and Cy 3. TAMRA might be excited using 543 or 546 nm laser line and displays good optical property. Hydrazides can label aldehyde and ketone through reductive amination reaction to form an imine linkage. The main labeling target for hydrazides are free reducing sugars on biomolecules, and prior to conjugation, primary and secondary alcohols on polysaccharide and glycoprotein are usually oxidized to aldehyde and ketone.  We offer TAMRA hydrazide for labeling of polysaccharide, glycoprotein and other biomolecules bearing aldehyde or ketone. 


Fluorophore: TAMRA

Reactive group: Hydrazide

Excitation/Emission Max.(nm): 544/576 

Spectrally similar dyes: DyLight549, Cy3, ATTO550

Extinction coefficient: ≥ 86,000 cm-1M-1

CF280: missing

Appearance: Red Solid

Molecular Weight: 446.50 g/mol    

Solubility: DMF, DMSO

Storage conditions: -20 ℃, protect from light

Citation & Reference

1. Troy A. Walton. Evaluation of New Linkers and Synthetic Methods for Internal Modified Oligonucleotides. Bioconjugate Chem 13.5 (2002): 1155-1158.

2. Kenneth J. Livak. Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic acid hybridization. PCR Methods Appl 4.6 (1995): 357-62.

3. Matthew H. Lyttle. A Tetramethyl Rhodamine (Tamra) Phosphoramidite Facilitates Solid-Phase-Supported Synthesis of 5‘-Tamra DNA. J. Org. Chem 65.26 (2000): 9033-9038.

4. Ravi Vinayak. A convenient, solid-phase coupling of rhodamine dye acids to 5′ amino-oligonucleotides. Tetrahedron Letters 40.43 (1999): 7611-7613.


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