TAMRA Amine

Catalog No. KWE1025
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1 mg $121.00
5 mg $279.00
25 mg $552.00
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Description

TAMRA Amine is an inactive form of bright yellow dye that used to generate a stable fluorescence signal in bioimaging. The maxima of Ex/Em values are at 553/575 nm, similar to that of DyLight 549, ATTO 550 and Cy 3. TAMRA might be excited using 543 or 546 nm laser line and displays good optical property. TAMRA amine can be coupled with carboxylic acids at small molecules or on biomolecules by standard amide bond coupling conditions or can be utilized as a reference standard for dye-conjugates.  

Specifications

Fluorophore: TAMRA

Functional group: Amine

Excitation/Emission Max.(nm): 553/575 

Spectrally similar dyes: DyLight549, Cy3, ATTO550

Extinction coefficient: ≥ 16,000 cm-1M-1

CF280: 0.5

Appearance: Red Solid

Molecular Weight: 474.55 g/mol    

Solubility: DMSO

Storage conditions: 4 ℃, protect from light




Citation & Reference

1. Troy A. Walton. Evaluation of New Linkers and Synthetic Methods for Internal Modified Oligonucleotides. Bioconjugate Chem 13.5 (2002): 1155-1158.

 

2. Kenneth J. Livak. Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic acid hybridization. PCR Methods Appl 4.6 (1995): 357-62.

 

3. Matthew H. Lyttle. A Tetramethyl Rhodamine (Tamra) Phosphoramidite Facilitates Solid-Phase-Supported Synthesis of 5‘-Tamra DNA. J. Org. Chem 65.26 (2000): 9033-9038.

 

4. Ravi Vinayak. A convenient, solid-phase coupling of rhodamine dye acids to 5′ amino-oligonucleotides. Tetrahedron Letters 40.43 (1999): 7611-7613.

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