Description

BioActs ECL Kit (Western Blotting Detection Kit) is a highly sensitive chemiluminescent substrate designed for the detection of horseradish peroxidase (HRP)-conjugated antibodies on Western blot membranes.

This kit provides strong signal intensity, low background, and a wide dynamic range, enabling detection of both abundant and low-expression proteins with high clarity.

The BioActs ECL Kit consists of two components: Reagent A and Reagent B, each provided in 50 mL volumes (total 100 mL).

These components are mixed immediately before use to generate a stable chemiluminescent signal suitable for both X-ray film and CCD-based imaging systems.

BioActs ECL Kit is optimized for rapid and reliable results across a variety of Western blot applications.

 

 

Specifications

 Application: Western blot detection using HRP-conjugated antibodies

 Detection Method: Chemiluminescence

 Sensitivity: High (detects low pg protein levels)

 Dynamic Range: Wide (for both high and low abundance proteins)

 Signal Duration: Up to 1 hour (optimal imaging within 5–30 minutes)

 Reagents: Reagent A – 50 mL, Reagent B – 50 mL

 Total Volume: 100 mL (sufficient for ~2,000 cm² membrane surface)

 Recommended Volume: 0.1–0.5 mL/cm² of membrane

 Storage Conditions: 2–8 °C, protect from light

 Shelf Life: 12 months from manufacturing date

 Compatible Imaging Systems: X-ray film, CCD-based digital imagers

 Mixing Ratio: 1:1 (Reagent A : Reagent B)

 

Citation & Reference

 

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