FSD Fluor™ 647 NHS ester is the new generation of amine reactive far-red fluorescent dye developed by BioActs’ cutting-edge technology displaying excellent optical property comparing to spectrally similar dyes. The fluorescence intensity after binding to biomolecules such as antibody, nucleotide, and protein is also excellent, thus FSD Fluor™ series is ideal for various biochemical and biological analytical applications. FSD dye is conceivably the best existent dye for single-molecular detection of bioconjugates for fluorescence correlation spectroscopy and for fluorescence polarization measurements. The maxima of Ex/Em values are at 651/667 nm, similar to that of Alexa 647, Cy5 and DyLight 650. FSD 647 might be excited using 593 or 633 nm laser lines and displays excellent optical property. FSD 647 can be conjugated to low-abundance biomolecules with great sensitivity and high molar ratios, allowing sensitive detection. NHS esters readily react with amine-modified oligonucleotides or amino groups of proteins, i.e. the ε-amino groups of lysine or the amine terminus of nucleotides to form a chemically stable amide bond between dye and the biomolecule. We offer FSD Fluor™ 647 NHS ester for labeling of antibodies, peptides, proteins, ligands, and amplification substrates optimized for cellular labeling and detection.
1. Xu, Peisheng. Zwitterionic chitosan derivatives for pH-sensitive stealth coating. Biomacromolecules 11.9 (2010): 2352-2358.
2. Ibrahim, Basma M. A strategy to deliver genes to cystic fibrosis lungs: a battle with environment. Journal of controlled release 155.2 (2011): 289-295.
3. Oh, Keun Sang. Accurate sequential detection of primary tumor and metastatic lymphatics using a temperature-induced phase transition nanoparticulate system. International journal of nanomedicine 9 (2014): 2955.
4. Yhee, Ji Young. Tumor-targeting transferrin nanoparticles for systemic polymerized siRNA delivery in tumor-bearing mice. Bioconjugate chemistry 24.11 (2013): 1850-1860.
5. Yoon, Hong Yeol. Glycol chitosan nanoparticles as specialized cancer therapeutic vehicles: Sequential delivery of doxorubicin and Bcl-2 siRNA. Scientific reports 4 (2014).
6. Ryu, Ju Hee. Early diagnosis of arthritis in mice with collagen?induced arthritis, using a fluorogenic matrix metalloproteinase 3–specific polymeric probe. Arthritis & Rheumatism 63.12 (2011): 3824-3832.
7. Hollis, Christin P. In vivo investigation of hybrid paclitaxel nanocrystals with dual fluorescent probes for cancer theranostics. Pharmaceutical research 31.6 (2014): 1450-1459.
8. Koo, Heebeom. The movement of self-assembled amphiphilic polymeric nanoparticles in the vitreous and retina after intravitreal injection. Biomaterials 33.12 (2012): 3485-3493.
9. Zhu, Lei. Real-time monitoring of caspase cascade activation in living cells. Journal of controlled release 163.1 (2012): 55-62.
10. Yoon, Hong Yeol. Bioreducible hyaluronic acid conjugates as siRNA carrier for tumor targeting. Journal of Controlled Release 172.3 (2013): 653-661.
11. Yhee, Ji Young. Cancer-targeted MDR-1 siRNA delivery using self-cross-linked glycol chitosan nanoparticles to overcome drug resistance. Journal of Controlled Release 198 (2015): 1-9.
12. Park, Jin Woo. Wide-Ranged Fluorescent Molecular Weight Size Markers for Electrophoresis. Bulletin of the Korean Chemical Society 34.1 (2013): 29-30.
13. Huang, Xinglu. Multiplex Imaging of an Intracellular Proteolytic Cascade by using a Broad?Spectrum Nanoquencher. Angewandte Chemie International Edition 51.7 (2012): 1625-1630.