FSD Fluor™ 555 NHS ester is the new generation of amine reactive bright yellow dye developed by BioActs’ cutting-edge technology displaying excellent optical property comparing to spectrally similar dyes. The fluorescence intensity after binding to biomolecules such as antibody, nucleotide, and protein is also excellent, thus FSD Fluor™ series is ideal for various biochemical and biological analytical applications. FSD dye is conceivably the best existent dye for single-molecular detection of bioconjugates for fluorescence correlation spectroscopy and for fluorescence polarization measurements. The maxima of Ex/Em values are at 554/565 nm, similar to that of Alexa 555, DyLight 549, ATTO 550 and Cy3. FSD 555 might be excited using 532, 543, 546 or 555 nm laser lines and displays excellent optical property. FSD 555 can be conjugated to low-abundance biomolecules with great sensitivity and high molar ratios, allowing sensitive detection. NHS esters readily react with amine-modified oligonucleotides or amino groups of proteins, i.e. the ε-amino groups of lysine or the amine terminus of nucleotides to form a chemically stable amide bond between dye and the biomolecule. We offer FSD Fluor™ 555 NHS ester for labeling of antibodies, peptides, proteins, ligands, and amplification substrates optimized for cellular labeling and detection.
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