Flamma® Fluors 675 Azide is a copper (I)-catalyzed azide-alkyne cycloaddition (CuAAC) reagent of near infrared (NIR) fluorescent dye induced from benzindocyanine structure and used to generate a stable fluorescence signal in bioimaging. The azide reactive group is connected to the dye through an amino propyl linkage. The maxima of Ex/Em values are at 674/691 nm, similar to that of Alexa 680, Cy5.5, IRDye 680LT and DyLight 680. Flamma 675 might be excited using 633 nm laser line and the emission occurs at biological tissue permeable NIR region. Flamma 675 azide couples with an alkyne to form 1,4-disubstituted 1,2,3-triazole inside of living systems without interfering native biochemical processes. Prior to perform CuAAC, the alkyne functionality should be introduced onto counterpart biomolecule by means of chemical or genetic modification. We offer Flamma Fluors 675 Azide as a click chemistry reagent dye for cellular imaging and nucleotide functionalization.
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