Flamma® Fluors 648 Azide is a copper (I)-catalyzed azide-alkyne cycloaddition (CuAAC) reagent of far-red dye induced from cyanine structure and used to generate a stable fluorescence signal in bioimaging. The azide reactive group is connected to the dye through an amino propyl linkage. The maxima of Ex/Em values are at 648/663 nm, similar to that of Alexa 647, Cy5 and DyLight 650. Flamma 648 might be excited using 593 or 633 nm laser lines and displays excellent optical property. Flamma 648 azide couples with an alkyne to form 1,4-disubstituted 1,2,3-triazole inside of living systems without interfering native biochemical processes. Prior to perform CuAAC, the alkyne functionality should be introduced onto counterpart biomolecule by means of chemical or genetic modification. We offer Flamma Fluors 648 Azide as a click chemistry reagent dye for cellular imaging and nucleotide functionalization.
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