Flamma® Fluors 774 ADIBO is a strain-promoted azide-alkyne cycloaddition (SPAAC) reagent of near infrared (NIR) fluorescent dye and used to generate a stable fluorescence signal in bioimaging. The maxima of Ex/Em values are at 774/800 nm, similar to that of IRDye 800, Cy7.5 and CF770. Flamma® 774 might be excited using 750 or 785 nm laser line or dye-pumped laser excitation and the emission occurs at NIR region. Flamma 774 ADIBO couples with an azide to form 1,4-disubstituted 1,2,3-triazole inside of living systems without using any coupling reagents or catalyst nor interfering native biochemical processes. Prior to perform SPAAC, the azide functionality should be introduced onto counterpart biomolecule by means of chemical or genetic modification. We offer Flamma Fluors 774 ADIBO as a click chemistry reagent dye for cellular imaging and nucleotide functionalization.
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