Flamma® Fluors 675 PEG4-alkyne is a copper (I)-catalyzed azide-alkyne cycloaddition (CuAAC) reagent of near infrared (NIR) fluorescent dye induced from benzindocyanine structure and used to generate a stable fluorescence signal in bioimaging. The alkyne reactive group is connected to the dye through a tetraethylene glycol spacer. The maxima of Ex/Em values are at 675/691 nm, similar to that of Alexa 680, Cy5.5, IRDye 680LT and DyLight 680. Flamma 675 might be excited using 633 nm laser line and the emission occurs at biological tissue permeable NIR region. Flamma 675 PEG4-alkyne couples with an azide to form 1,4-disubstituted 1,2,3-triazole inside of living systems without interfering native biochemical processes. Prior to perform CuAAC, the azide functionality should be introduced onto counterpart biomolecule by means of chemical or genetic modification. We offer Flamma Fluors 675 PEG4-alkyne as a click chemistry reagent dye for cellular imaging and nucleotide functionalization. 


Fluorophore: Flamma® Fluors 675

Reactive group: PEG4-alkyne

Excitation/Emission Max.(nm): 675/691 

Spectrally similar dyes: Alexa680, DyLight680, Cy5.5, IRDye680LT

Extinction coefficient: ≥ 157,000 cm-1M-1

CF280: 0.1

Appearance: Blue Solid

Molecular Weight: 1158.38 g/mol    

Solubility: DMF, DMSO

Storage conditions: -20 ℃, protect from light 

Citation & Reference

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