Flamma® Fluors 749 Vinylsulfone is pH insensitive reactive form of near infrared (NIR) fluorescent dye induced from cyanine structure and used to generate a stable fluorescence signal in bioimaging. The maxima of Ex/Em values are at 749/774 nm, similar to that of Alexa 750, Cy7, IRDye 750 and DyLight 755. Flamma 749 might be excited using 750 nm laser line or dye-pumped laser excitation and the emission occurs at biological tissue permeable NIR region. Flamma 749-conjugated primary and secondary antibody are used as molecular probes for in vitro imaging and other fluorescence detection methods. Vinylsulfones readily react with primary amines of amino-modified oligonucleotides or of proteins to form a stable amino linkage between dye and the biomolecule. We offer Flamma Fluors 749 dye for labeling of antibodies, peptides, proteins, ligands and in vivo NIR imaging.
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