BioActs offers the full spectrum of fluorescent dyes such as FSD FluorTM, Flamma® Fluors, ICG, and Other Dyes,
and also offers Fluorescent Quenchers, Crosslinkers, Fluorescent Antibodies, Bioprobes,
and Microspheres, Magnetic Beads, Dye Labeling Kit, etc.

Luciferase Assay

Luciferase is a generic term for the class of oxidative enzymes that produce bioluminescence and usually distinguished from a photoprotein. Luciferases are widely used as reporters and fluorescent proteins in biotechnology, in microscopy, and for many of other applications. Unlike fluorescent proteins, luciferases do not require an external light source, but their activation requires addition of luciferin. The most famous one of these enzymes is firefly luciferase. Fireflies are able to emit light via a chemical reaction, which luciferin is converted to oxyluciferin by luciferase. Luciferase assay has broad applications in various fields of cell and molecular biology.

Luciferase assay is commonly used the tool to study gene expression at the transcriptional level. Due to the convenience, cost-effectiveness and the ability to generate instantaneous results, the assay is widely utilized in biochemistry researches. This reaction is highly energetically efficient that nearly all the energy input into the reaction is rapidly converted to light, which makes it extremely sensitive. Luciferase assay can be used to study gene expression as well as other cellular components and events that are involved in gene regulation. The extremely sensitive assay allows to quantify small changes in transcription, and the availability to immediately obtain the assay results makes the assay more appealing.

The assay is also utilized in the whole animal imaging, a powerful technique for studying cell populations in live animals. Different types of cells (e.g. bone marrow stem cells, T-cells) can be engineered to express a luciferase allowing their non-invasive visualization inside a live animal using a sensitive charge-couple device camera. This technique has been used to follow tumorigenesis and response of tumors to treatment in animal models. However, environmental factors and therapeutic interferences may cause some discrepancies between tumor burden and bioluminescence intensity in relation to changes in proliferative activity. The intensity of the signal measured by in vivo imaging may depend on various factors, such as luciferin absorption through the peritoneum, blood flow, and cell membrane permeability, availability of co-factors, intracellular pH and transparency of overlying tissue, etc.

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