FSD Fluor™ 647 Phalloidin

Catalog No. RCS2414
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Description

 FSD Fluor™ 647 Phalloidin is a filamentous actin (F-actin) labeling probe that phalloidin is conjugated with our bright, photostable FSD 647. Phalloidin is a rigid bicyclic peptide toxin isolated from Amanita phalloides mushroom that commonly used in imaging applications to selective label F-actin. Phalloidin binds stoichiometrically with actin. Fluorescent phalloidin conjugates are not permeable to most live cells, thus they should be used to detect cells with compromised membranes. However, fluorescent labeled phalloidins may penetrate the membranes of certain hypoxic cells. We offer FSD Fluor™ 647 Phalloidin for the labeling and quantitative analysis of F-actin in formaldehyde-fixed and permeabilized tissue sections, cell cultures, and cell-free experiments.

Specifications

 Fluorophore: FSD Fluor™ 647

Application: Actin, Cytoskeleton imaging

Excitation/Emission Max.(nm): 651/667 nm

Storage conditions: -20 ℃, protect from light

Citation & Reference

1. Lynen F, Wieland U (18 November 1937). "Uber die Giftstoffe des Knollenblätterpilzes. IV". Justus Liebigs Annalen der Chemie (in German). 533 (1): 93–117. 

 

2. Wieland T, Schon W (16 January 1955). "Über die Giftstoffe des grünen Knollenblätterpilzes X. Mitteilung. Die Konstitution des Phalloidins". Justus Liebigs Annalen der Chemie. 593 (2): 157–178.

 

3. Immunohistochemistry: Basics and Methods. Springer Science & Business Media. 2010. pp. 92–3.


4. Walton JD; Hallen-Adams He; Luo H (4 August 2010). "Ribosomal biosynthesis of the cyclic peptide toxins of Amanita mushrooms". Peptide Science. 94 (5): 659–654.

 

5. Anderson MO, Shelat AA, Kiplan Guy R (16 April 2005). "A solid-phase approach to the phallotoxins: total synthesis of [ala7]-phalloidin". J. Org. Chem. 70 (12): 4578–84.

 

6. Wieland T (1963). "Chemical and toxicological studies with cyclopeptides of Amanita phalloides". Pure and Applied Chemistry. 3 (6): 339–350.

 

7. Schröder, Eberhard; Lübke, Klaus (2014). The Peptides, Volume II: Synthesis, Occurrence, and Action of Biologically Active Polypeptides. Elsevier. p. 475.

 

8. Cooper JA (October 1987). "Effects of cytochalasin and phalloidin on actin". J. Cell Biol. 105 (4): 1473–8.


9. Barden JA, Miki M, Hambly BD, Dos Remedios CG (February 1987). "Localization of the phalloidin and nucleotide-binding sites on actin". Eur. J. Biochem. 162 (3): 583–8.

 

10. Wehland J, Osborn M, Weber K (December 1977). "Phalloidin-induced actin polymerization in the cytoplasm of cultured cells interferes with cell locomotion and growth". Proc. Natl. Acad. Sci. U.S.A. 74 (12): 5613–7.

 

11. Capani F, Deerinck TJ, Ellisman MH, Bushong E, Bobik M, Martone ME (1 November 2001). "Phalloidin-eosin followed by photo-oxidation: a novel method for localizing F-actin at the light and electron microscopic levels". J. Histochem.Cytochem. 49 (11): 1351–61.

 

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