Description

Goat anti-rat IgG, FSD™ 750 is a fluorescence conjugated secondary antibody that displays excellent optical imaging with low cross reactivity. Anti-rat secondary antibodies display specificity for rat IgG and are useful for the detection of specific target. Since multiple secondary antibodies can bind to a single primary antibody, Goat anti-rat IgG, FSD™ 750 might provide the great sensitivity in signal amplification, visualize low abundant targets and reduce experimental time. FSD Fluor™ is a new generation of dye series with superb fluorescence intensity and high quantum yield comparing to traditional dyes. FSD™ 750 might be excited using 750 nm laser line and displays excellent optical property. We offer Goat anti-rat IgG, FSD™ 750 as a suitable fluorescent molecular probe for many biological experiments such as fluorescence microscopy, flow cytometry, microplate assays, protein and nucleic acid blots, in situ hybridization, etc.

 

 

Applications

Notes

Western Blot (WB)

0.1-0.4 µg/mL

Immunohistochemistry (IHC)

Assay-dependent

Immunocytochemistry (ICC/IF)

1-10 µg/mL

Flow Cytometry (Flow)

1-10 µg/mL

 

 

Specifications

Conjugate: FSD 750

Species reactivity: Rat

Target: IgG

Excitation/Emission Max.(nm): 751/774 

Host/Isotype: Goat/IgG

Type: Secondary antibody

Antibody form: Whole antibody

Form: Liquid

Concentration: 2 mg/mL

Storage buffer: PBS (pH 7.4)    

Contains: 1.5% BSA, 5 mM sodium azide    

Storage conditions: 4 ℃, protect from light

Citation & Reference

1. Agustina, Lia. Visualization of the physical and functional interaction between hMYH and hRad9 by Dronpa bimolecular fluorescence complementation. BMC molecular biology 15.1 (2014): 1.



2. Lee, Cheol-Jung. Magnolin inhibits cell migration and invasion by targeting the ERKs/RSK2 signaling pathway. BMC cancer 15.1 (2015): 1.



3. Promoter- and RNA polymerase II-dependent hsp-16 gene association with nuclear pores in Caenorhabditis elegans. Rohner S,Kalck V,Wang X,Ikegami K,Lieb JD,Gasser SM,Meister P The Journal of cell biology (200:589) 



4. BRAG2/GEP100/IQSec1 interacts with clathrin and regulates α5β1 integrin endocytosis through activation of ADP ribosylation factor 5 (Arf5). Moravec R,Conger KK,D'Souza R,Allison AB,Casanova JE The Journal of biological chemistry (287:31138)

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