Description

 Biotin, Flamma® 552 is a fluorescent biotin that Flamma® 552 dye is covalently attached to biotin. Biotin, a 244 dalton vitamin found in all living cells, binds to avidin and streptavidin in high affinity. In biotechnology, biotin conjugated antibodies, enzymes, small molecules or reporters are used to form complex with avidin/streptavidin. Flamma® 552 is a bright yellow fluorescent dye. Flamma® 552 might be excited using 532, 543, 546 or 555 nm laser line. Fluorescence–conjugated biotins have been utilized in various applications such as cell surface labeling, ELISA, immunohistochemistry, affinity purification, FACS, EMSA, etc. The bond formation between biotin and avidin/streptavidin is rapid, and the binding is stable at high temperature, in a wide range of pH and denaturing agents. BioActs offers Biotin, Flamma® 552 as an effective detecting and analytic probe for diverse applications in medical diagnostics, in vitro, in vivo and animal model study.

Specifications

Fluorophore label: Flamma® 552

Reactive group: Biotin

Reacting toward: Avidin / Streptavidin

Excitation/Emission Max.(nm): 550/565

Molecular weight: 913.18 g/mol 

Storage conditions: 4 ℃, protect from light

Citation & Reference

 

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