Flamma Fluors 552NA NHS ester is a reactive form of bright yellow fluorescent dye that analogous to cyanine 3 and used to generate a stable fluorescence signal in bioimaging. The maxima of Ex/Em values are at 553/570 nm, similar to that of Cy 3, Quasar 570. Flamma 552 might be excited using 532, 543, 546 or 555 nm laser lines and displays excellent optical property. Flamma 552NA dye can be conjugated into low-abundance biomolecules or incorporated into oligonucleotide synthesis as a fluorescence label. NHS esters readily react with amino groups of proteins, i.e. the ε-amino groups of lysine, or amine terminus of modified nucleotides to form a chemically stable amide bond between dye and the biomolecule. We offer Flamma Fluors 552NA NHS ester for labeling of biomolecules and amino-modified oligonucleotide in solid phase oligonucleotide synthesis.
1. Xu, Peisheng. Zwitterionic chitosan derivatives for pH-sensitive stealth coating. Biomacromolecules 11.9 (2010): 2352-2358.
2. Ibrahim, Basma M. A strategy to deliver genes to cystic fibrosis lungs: a battle with environment. Journal of controlled release 155.2 (2011): 289-295.
3. Oh, Keun Sang. Accurate sequential detection of primary tumor and metastatic lymphatics using a temperature-induced phase transition nanoparticulate system. International journal of nanomedicine 9 (2014): 2955.
4. Yhee, Ji Young. Tumor-targeting transferrin nanoparticles for systemic polymerized siRNA delivery in tumor-bearing mice. Bioconjugate chemistry 24.11 (2013): 1850-1860.
5. Yoon, Hong Yeol. Glycol chitosan nanoparticles as specialized cancer therapeutic vehicles: Sequential delivery of doxorubicin and Bcl-2 siRNA. Scientific reports 4 (2014).
6. Ryu, Ju Hee. Early diagnosis of arthritis in mice with collagen?induced arthritis, using a fluorogenic matrix metalloproteinase 3–specific polymeric probe. Arthritis & Rheumatism 63.12 (2011): 3824-3832.
7. Hollis, Christin P. In vivo investigation of hybrid paclitaxel nanocrystals with dual fluorescent probes for cancer theranostics. Pharmaceutical research 31.6 (2014): 1450-1459.
8. Koo, Heebeom. The movement of self-assembled amphiphilic polymeric nanoparticles in the vitreous and retina after intravitreal injection. Biomaterials 33.12 (2012): 3485-3493.
9. Zhu, Lei. Real-time monitoring of caspase cascade activation in living cells. Journal of controlled release 163.1 (2012): 55-62.
10. Yoon, Hong Yeol. Bioreducible hyaluronic acid conjugates as siRNA carrier for tumor targeting. Journal of Controlled Release 172.3 (2013): 653-661.
11. Yhee, Ji Young. Cancer-targeted MDR-1 siRNA delivery using self-cross-linked glycol chitosan nanoparticles to overcome drug resistance. Journal of Controlled Release 198 (2015): 1-9.
12. Park, Jin Woo. Wide-Ranged Fluorescent Molecular Weight Size Markers for Electrophoresis. Bulletin of the Korean Chemical Society 34.1 (2013): 29-30.
13. Huang, Xinglu. Multiplex Imaging of an Intracellular Proteolytic Cascade by using a Broad?Spectrum Nanoquencher. Angewandte Chemie International Edition 51.7 (2012): 1625-1630.