TAMRA PEG4-Alkyne

Catalog No. KWG1025
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1 mg $121.00
5 mg $279.00
25 mg $552.00
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Description

TAMRA PEG4-Alkyne is a copper (I)-catalyzed azide-alkyne cycloaddition (CuAAC) reagent of bright yellow dye that used to generate a stable fluorescence signal in bioimaging. The maxima of Ex/Em values are at 543/575 nm, similar to that of DyLight 549, ATTO 550 and Cy 3. TAMRA might be excited using 543 or 546 nm laser line and displays good optical property. The alkyne reactive group is connected to the dye through a tetraethylene glycol spacer. TAMRA PEG4-alkyne couples with an azide to form 1,4-disubstituted 1,2,3-triazole inside of living systems without interfering native biochemical processes. Prior to perform CuAAC, the azide functionality should be introduced onto counterpart biomolecule by means of chemical or genetic modification. We offer TAMRA PEG4-alkyne as a click chemistry reagent dye for cellular imaging and nucleotide functionalization. 

Specifications

Fluorophore: TAMRA

Reactive group: Alkyne

Excitation/Emission Max.(nm): 549/573 

Spectrally similar dyes: DyLight549, Cy3, ATTO550

Extinction coefficient: ≥ 83,000 cm-1M-1

Appearance: Red Solid

Molecular Weight: 645.74 g/mol    

Solubility: DMF

Storage conditions: -20 ℃, protect from light




Citation & Reference

1. Troy A. Walton. Evaluation of New Linkers and Synthetic Methods for Internal Modified Oligonucleotides. Bioconjugate Chem 13.5 (2002): 1155-1158.


2. Kenneth J. Livak. Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic acid hybridization. PCR Methods Appl 4.6 (1995): 357-62.


3. Matthew H. Lyttle. A Tetramethyl Rhodamine (Tamra) Phosphoramidite Facilitates Solid-Phase-Supported Synthesis of 5‘-Tamra DNA. J. Org. Chem 65.26 (2000): 9033-9038.


4. Ravi Vinayak. A convenient, solid-phase coupling of rhodamine dye acids to 5′ amino-oligonucleotides. Tetrahedron Letters 40.43 (1999): 7611-7613.

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