Description

TAMRA Isothiocyanate is a reactive form of bright yellow dye that used to generate a stable fluorescence signal in bioimaging. The maxima of Ex/Em values are at 553/575 nm, similar to that of DyLight 549, ATTO 550 and Cy 3. TAMRA might be excited using 543 or 546 nm laser line and displays good optical property. Isothiocyanates are moderately reactive but quite stable in water and most organic solvents. Isothiocyanates react to primary amine to form reasonably stable thiourea linkage. Whereas labeling of protein with NHS esters can typically be done at pH 8.3, conjugation for isothiocyanates usually require pH above 9. We offer TAMRA isothiocyanate for labeling of antibodies, peptides, proteins and oligonucleotide labeling and automated DNA sequencing applications. 

Specifications

Fluorophore: TAMRA

Reactive group: Isothiocyanate

Excitation/Emission Max.(nm): 553/575 

Spectrally similar dyes: DyLight549, Cy3, ATTO550

Extinction coefficient: ≥ 16,000 cm-1M-1

CF280: 0.96

Appearance: Red Solid

Molecular Weight: 445.54 g/mol    

Solubility: DMF

Storage conditions: -20 ℃, protect from light





Citation & Reference

1. Troy A. Walton. Evaluation of New Linkers and Synthetic Methods for Internal Modified Oligonucleotides. Bioconjugate Chem 13.5 (2002): 1155-1158.


2. Kenneth J. Livak. Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic acid hybridization. PCR Methods Appl 4.6 (1995): 357-62.


3. Matthew H. Lyttle. A Tetramethyl Rhodamine (Tamra) Phosphoramidite Facilitates Solid-Phase-Supported Synthesis of 5‘-Tamra DNA. J. Org. Chem 65.26 (2000): 9033-9038.


4. Ravi Vinayak. A convenient, solid-phase coupling of rhodamine dye acids to 5′ amino-oligonucleotides. Tetrahedron Letters 40.43 (1999): 7611-7613.

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