FAM PEG4-Alkyne

Catalog No. PWK1701
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1 mg $121.00
5 mg $279.00
25 mg $552.00
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Description

FAM PEG4-Alkyne is a copper (I)-catalyzed azide-alkyne cycloaddition (CuAAC) reagent of bright green dye used to generate a stable fluorescence signal in bioimaging. The maxima of Ex/Em values are at 494/522 nm, similar to that of Alexa 488 and Cy2. FAM might be excited using 488 nm laser line and displays good optical property. The alkyne reactive group is connected to the dye through a tetraethylene glycol spacer. FAM PEG4-alkyne couples with an azide to form 1,4-disubstituted 1,2,3-triazole inside of living systems without interfering native biochemical processes. Prior to perform CuAAC, the azide functionality should be introduced onto counterpart biomolecule by means of chemical or genetic modification. We offer FAM PEG4-alkyne as a click chemistry reagent dye for cellular imaging and nucleotide functionalization. 

Specifications

Fluorophore: FAM

Reactive group: Alkyne

Excitation/Emission Max.(nm): 494/522 

Spectrally similar dyes: Alexa488, Cy2

Extinction coefficient: ≥ 60,000 cm-1M-1

CF280: 0.17

Appearance: Yellow Solid

Molecular Weight: 591.61 g/mol    

Solubility: DMF

Storage conditions: -20 ℃, protect from light




Citation & Reference

1. PYARE L. KHANNAA. 4',5'-Dimethoxy-6-carboxyfluorescein: a novel dipole-dipole coupled fluorescence energy transfer acceptor useful for fluorescence immunoassays. Anal Biochem 108.1 (1980): 156-61.


2. Torimura M. Fluorescence-quenching phenomenon by photoinduced electron transfer between a fluorescent dye and a nucleotide base. Anal Sci 17.1 (2001): 155-60.


3. Sylvie Soulie-Begu. In-vivo pharmacokinetic study of two fluorescein derivatives by fluorescence spectroscopy. Optical Biopsies 2627 (1995).


4. Danny van Lierop. Positively charged silver nanoparticles and their effect on Surfaceenhanced Raman scattering of dye-labelled oligonucleotides. Chem. Commun 48 (2012): 8192-8194.


5. Pete Theisen. Fluorescent Dye Phosphoramidite Labelling of Oligonucleotides. Tetrahedron Letters 33.35 (1992): 5033-5036.

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