FAM Vinylsulfone

Catalog No. CWA1020
PACKING UNIT

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1 mg $121.00
5 mg $278.25
25 mg $551.25
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Description

FAM Vinylsulfone is pH insensitive reactive form of bright green dye used to generate a stable fluorescence signal in bioimaging. Vinylsulfone reactive group, developed by BioActs’ leading technology, is stable in a wide range of pH and at the high temperature. The maxima of Ex/Em values are at 494/518 nm, similar to that of Alexa 488 and Cy2. FAM might be excited using 488 nm laser line and displays good optical property. Vinylsulfones readily react with primary amines of amino-modified oligonucleotides or of proteins to form a stable amino linkage between dye and the biomolecule.We offer FAM vinylsulfone for labeling of antibodies, peptides, proteins and nucleotide. 

Specifications

Fluorophore: FAM

Reactive group: Vinylsulfone

Excitation/Emission Max.(nm): 494/520 

Spectrally similar dyes: Alexa488, Cy2

Extinction coefficient: ≥ 58,000 cm-1M-1

CF280: 0.2

Appearance: Yellow Solid

Molecular Weight: 495.1 g/mol    

Solubility: DMF, DMSO

Storage conditions: 4 ℃, protect from light



Citation & Reference

1. PYARE L. KHANNAA. 4',5'-Dimethoxy-6-carboxyfluorescein: a novel dipole-dipole coupled fluorescence energy transfer acceptor useful for fluorescence immunoassays. Anal Biochem 108.1 (1980): 156-61.


2. Torimura M. Fluorescence-quenching phenomenon by photoinduced electron transfer between a fluorescent dye and a nucleotide base. Anal Sci 17.1 (2001): 155-60.


3. Sylvie Soulie-Begu. In-vivo pharmacokinetic study of two fluorescein derivatives by fluorescence spectroscopy. Optical Biopsies 2627 (1995).


4. Danny van Lierop. Positively charged silver nanoparticles and their effect on Surfaceenhanced Raman scattering of dye-labelled oligonucleotides. Chem. Commun 48 (2012): 8192-8194.


5. Pete Theisen. Fluorescent Dye Phosphoramidite Labelling of Oligonucleotides. Tetrahedron Letters 33.35 (1992): 5033-5036.

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