qFlamma® Black01 Amine is an inactive form of universal dark quencher that the range of absorption covers from 400 to 800 nm with no native fluorescence. Black01 quencher displays high absorption coefficient, superb stability and aqueous solubility. The primary amine is attached to Black01 quencher through a spacer. qFlamma® Black01 amine can be coupled with carboxylic acids at a probe or on biomolecules by standard amide bond coupling conditions or can be utilized as a reference standard for fluorophore-quencher pair probes.