qFlamma® Black01 NHS ester

Catalog No. QWS1001
PACKING UNIT

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1 mg $121.00
5 mg $280.00
25 mg $552.00
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Description

qFlamma® Black01 NHS ester is an amine reactive form of universal dark quencher that the range of absorption covers from 400 to 800 nm with no native fluorescence. Black01 quencher displays high absorption coefficient, superb stability and aqueous solubility. NHS esters readily react with or amino groups of probes, peptides or the amine terminus of nucleotides to form a chemically stable amide bond between the quencher and the biomolecule. We offer qFlamma® Black01 NHS ester as a universal quencher in the construction of a variety of hybridization and custom-synthesized activatable probes for multiplexed bio-analysis, PCR, nucleic acid hybridization, etc.  

Specifications

Functionality: Dark quencher

Reactive group: NHS ester

Absorption max. (nm): 484

Absorption range (nm): 400 ~ 800

Extinction coefficient: 21,000 cm-1M-1

CF280: 0.8

Appearance: Green Solid

Molecular Weight: 818.83 g/mol

Solubility: Water, DMF, DMSO

Storage conditions: -20 ℃, protect from light



Citation & Reference

1. Arnaud Chevalier. Bioconjugatable Azo-Based Dark-Quencher Dyes: Synthesis and Application to Protease-Activatable Far-Red Fluorescent Probes. Chemistry 19.5 (2013): 1686-99.

 

2. Arnaud Chevalier. Universal Dark Quencher Based on “Clicked” Spectrally Distinct Azo Dyes. Org. Lett 2013 15.23 (2013): 6082–6085.


3. Arnaud Chevalier. Straightforward synthesis of bioconjugatable azo dyes. Part 1: Black Hole Quencher-1 (BHQ-1) scaffold. Tetrahedron Letters 55.50 (2014): 6759-6763.

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