Flamma® Fluors 581 PEG4-alkyne is a copper (I)-catalyzed azide-alkyne cycloaddition (CuAAC) reagent of orange dye induced from benzindocyanine structure and used to generate a stable fluorescence signal in bioimaging. The alkyne reactive group is connected to the dye through a tetraethylene glycol spacer. The maxima of Ex/Em values are at 578/593 nm, similar to that of Alexa 594 and DyLight 594. Flamma 581 might be excited using 561, 568 or 578 nm laser lines and displays excellent optical property. Flamma 581 PEG4-alkyne couples with an azide to form 1,4-disubstituted 1,2,3-triazole inside of living systems without interfering native biochemical processes. Prior to perform CuAAC, the azide functionality should be introduced onto counterpart biomolecule by means of chemical or genetic modification. We offer Flamma Fluors PEG4-581 alkyne as a click chemistry reagent dye for cellular imaging and nucleotide functionalization. 


Fluorophore: Flamma® Fluors 581

Reactive group: PEG4-alkyne

Excitation/Emission Max.(nm): 578/593 

Spectrally similar dyes: Alexa594, DyLight594

Extinction coefficient: ≥ 109,000 cm-1M-1

CF280: 0.18

Appearance: Purple Solid

Molecular Weight: 1132.34 g/mol    

Solubility: DMF, DMSO

Storage conditions: -20 ℃, protect from light

Citation & Reference

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