Description

Flamma® Fluors 496 Hydrazide is a reactive form of bright green dye induced from fluorescein structure and used to generate a stable fluorescence signal in bioimaging. The maxima of Ex/Em values are at 496/520 nm, similar to that of Fluorescein. Flamma 496 might be excited using 488 nm laser line and displays excellent optical property. Hydrazides can label aldehyde and ketone through reductive amination reaction to form an imine linkage. The main labeling target for hydrazides are free reducing sugars on biomolecules, and prior to conjugation, primary and secondary alcohols on polysaccharide and glycoprotein are usually oxidized to aldehyde and ketone.  We offer Flamma Fluors 496 hydrazide for labeling of polysaccharide, glycoprotein and other biomolecules bearing aldehyde or ketone. 

Specifications

Fluorophore: Flamma® Fluors 496

Reactive group: Hydrazide

Excitation/Emission Max.(nm): 496/520 

Spectrally similar dyes: Alexa488, FITC, Cy2

Extinction coefficient: ≥ 43,000 cm-1M-1

CF280: 0.12

Appearance: Yellow to Orange Solid

Molecular Weight: 428.34 g/mol    

Solubility: DMF, DMSO

Storage conditions: -20 ℃, protect from light




Citation & Reference

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