Description

Flamma® Fluors 749 Vinylsulfone is pH insensitive reactive form of near infrared (NIR) fluorescent dye induced from cyanine structure and used to generate a stable fluorescence signal in bioimaging. The maxima of Ex/Em values are at 749/774 nm, similar to that of Alexa 750, Cy7, IRDye 750 and DyLight 755. Flamma 749 might be excited using 750 nm laser line or dye-pumped laser excitation and the emission occurs at biological tissue permeable NIR region. Flamma 749-conjugated primary and secondary antibody are used as molecular probes for in vitro imaging and other fluorescence detection methods. Vinylsulfones readily react with primary amines of amino-modified oligonucleotides or of proteins to form a stable amino linkage between dye and the biomolecule. We offer Flamma Fluors 749 dye for labeling of antibodies, peptides, proteins, ligands and in vivo NIR imaging.  

Specifications

Fluorophore: Flamma® Fluors 749

Reactive group: Vinylsulfone

Excitation/Emission Max.(nm): 749/774

Spectrally similar dyes: Alexa750, DyLight755, Cy7, IRDye750

Extinction coefficient: ≥ 200,000 cm-1M-1

CF280: 0.03

Appearance: Green Solid

Molecular Weight: 828.07 g/mol

Solubility: DMF, DMSO

Storage conditions: 4 ℃, protect from light



Citation & Reference

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21. Huang, Xinglu. Multiplex Imaging of an Intracellular Proteolytic Cascade by using a Broad?Spectrum Nanoquencher. Angewandte Chemie International Edition 51.7 (2012): 1625-1630. 

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