Flamma® Fluors 749 NHS ester is a reactive form of near infrared (NIR) fluorescent dye induced from cyanine structure and used to generate a stable fluorescence signal in bioimaging. The maxima of Ex/Em values are at 749/774 nm, similar to that of Alexa 750, Cy7.5, IRDye 750 and Dylight 755. Flamma 749 might be excited using the 750 nm laser line or dye-pumped laser excitation and the emission occurs at biological tissue permeable NIR region. Flamma 749-conjugated primary and secondary antibody are used as molecular probes for in vitro imaging and other fluorescence detection methods. NHS esters readily react with amine-modified oligonucleotides or amino groups of proteins, i.e. the ε-amino groups of lysine or the amine terminus of nucleotides to form a chemically stable amide bond between dye and the biomolecule. We offer Flamma Fluors 749 dye for labeling of antibodies, peptides, proteins, ligands, and amplification substrates optimized for in vitro imaging.
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