Description

Flamma® Fluors 774 Sulfo-NHS ester is a reactive form of near infrared (NIR) fluorescent dye and used to generate a stable fluorescence signal with high signal-to-noise ratio. The maxima of Ex/Em values are at 774/800 nm, similar to that of IRDye 800, Cy7.5 and CF770. Flamma 774 might be excited using 750 nm laser line or dye-pumped laser excitation and the emission occurs at NIR region. Flamma Fluors 774 is ideal for protein, antibody and nucleic acid labeling for in vitro imaging and other fluorescence detection methods. Sulfo-NHS esters have higher water solubility than NHS esters, thus they do not need organic co-solvent and readily react with amine-modified oligonucleotides or amino groups of proteins, i.e. the ε-amino groups of lysine or the amine terminus of nucleotides to form a stable amide bond between dye and the biomolecule. We offer Flamma Fluors 774 Sulfo-NHS ester for labeling of antibodies, peptides, proteins, ligands, and in vivo NIR imaging.  

Specifications

Fluorophore: Flamma® Fluors 774

Reactive group: Sulfo-NHS ester

Excitation/Emission Max.(nm): 774/800 

Spectrally similar dyes: Cy7.5, DyLight800, IRDye800

Extinction coefficient: ≥ 182,000 cm-1M-1

CF280: 0.1

Appearance: Green Solid

Molecular Weight: 1106 g/mol   

Solubility: DMF, DMSO

Storage conditions: -20 ℃, protect from light

Citation & Reference

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